《植物生理学报》 2010, 46(4): 335-340

高山离子芥 MAP 激酶基因 CbMAPK3 的克隆

张腾国¹, 张艳¹, 王娟¹, 杨宁¹, 安黎哲^2,*

1 西北师范大学生命科学学院, 兰州 730070; 2 兰州大学生命科学学院, 兰州 730000

通信作者：安黎哲；E-mail: lizhean@lzu.edu.cn；Tel: 0931-7971722

摘　要：

从高山离子芥中克隆得到了一种新的 MAPK 基因的 cDNA CbMAPK3, 全长 1 419 bp, 其中包括 1 110 bp 的开放阅读 框、91 bp 的 5' 非翻译区(5'UTR)和 218 bp 的 3' 非翻译区(3'UTR)。CbMAPK3 基因编码 369 个氨基酸的蛋白质, 分子量为42.5 kDa, 等电点为 5.79, 含有 MAP 激酶所具有的 11 个保守序列区以及 MAP 激酶的磷酸化位点 TEY 基序。CbMAPK3蛋白与响应环境胁迫有关的植物 MAPK 亚类有很高的同源性。半定量分析表明, 该基因表达受低温胁迫诱导。

关键词：高山离子芥; MAPK 基因; 分子克隆

收稿：2009-11-16   修定：2010-03-02

资助：国家自然科学基金(30960065 和90302010)和西北师范大学知识与科技创新项目(nwnu-kjcxgc-03-76 和nwnukjcxgc-03-49)。

Molecular Cloning of a Novel MAP Kinase Gene CbMAPK3 in Chorispora bungeana

ZHANG Teng-Guo¹, ZHANG Yan¹, WANG Juan¹, YANG Ning¹, AN Li-Zhe^2,*

¹School of Life Sciences, Northwest Normal University, Lanzhou 730070, China; ²College of Life Sciences, Lanzhou University,Lanzhou 730000, China

Corresponding author: AN Li-Zhe; E-mail: lizhean@lzu.edu.cn; Tel: 0931-7971722

Abstract:

A new MAPK cDNA CbMAPK3 was isolated from Chorispora bungeana. The cloned full-length cDNA was 1 419 bp, contained a 1 110-bp ORF, a 5'-untranslated region (UTR) of 91 bp, and a 3'-untranslated region (UTR) of 218 bp. CbMAPK3 encoded a protein of 369 amino acids with a deduced molecular weight of about 42.5 kDa and with a PI of 5.79. The protein contained all 11 conserved subdomains and the phosphorylation TEY motif of MAPK. The CbMAPK3 protein exhibited closest homology to the subgroup of plant MAPKs which were involved in environmental response. Analysis by semi-quantitative RT-PCR indicated that the expression of CbMAPK3 was increased in response to cold stress.

Key words: Chorispora bungeana; MAPK gene; molecular cloning